Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 3282

#SCPH06 I Botany

Natural selection results in different forms of plants which can survive in contaminated or uncontaminated soil. Type of natural selection is

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#Question id: 8921

#I Life Science/ Life Sciences Group – I-V

Evidence of which structure or characteristic would be most surprising to find among fossils of the Ediacaran fauna?

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#Question id: 2960

#SCPH01 Biochemistry

Which type of spindle microtubules are attached to chromosomes?

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#Question id: 11017

#I Life Science/ Life Sciences Group – I-V

Which of the following is most likely at the “J point” in an EKG of a patient with a damaged cardiac muscle?

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#Question id: 3653

#SCPH01 Biochemistry

Cyclobutane pyrimidine dimers are formed by -------------from adjacent pyrimidines on one strand by cyclization of the double-bonded C5 and C6carbon atoms of each base to give a cyclobutane ring.