Agarose gels are formed by suspending dry agarose in aqueous buffer, in which usually Agarose concentration between

#Question id: 1393

#SCPH28 | Zoology

In research on aging (both cellular aging and organismal aging), it has been found that aged cells do not progress through the cell cycle as they had previously. Which of the following, if found in cells or organisms as they age, would provide evidence that this is related to cell signalling?

#Question id: 14500

#SCPH01 Biochemistry

#Question id: 5243

#SCPH28 | Zoology

Auxotroph culture (His- and Lac-) culture of E. coli was mutagenised. On what media would one spread the mutagenised cells to select prototroph for both His+  and Lac+ cells?

#Question id: 19341

#SCPH28 | Zoology

#Question id: 13076

#SCPH05 I Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.