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#Question id: 13064
#SCPH01 Biochemistry
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following techniques doesn’t involve use of a secondary antibody?
TLS Online TPP Program
#Question id: 18720
#SCPH01 Biochemistry
The derivatives of M13 vector are exact counterparts of _________
TLS Online TPP Program
#Question id: 5542
#I Life Science/ Life Sciences Group – I-V
One stage of cleavage is the morula stage which is:
TLS Online TPP Program
#Question id: 6986
#SCPH01 Biochemistry
The use of segments as a developmental strategy in vertebrates is evident in:
TLS Online TPP Program
#Question id: 27996
#Research Methodology
Most ex post facto research projects are used for descriptive studies in which the researcher seeks to measure item such as_____
a) Frequency of shopping
b) Preferences of people or similar data
c) manipulate variables
d) any form of manipulation