TLS Online TPP Program
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TLS Online TPP Program
#Question id: 2136
#SCPH06 I Botany
When a bacterium such as E. coli is shifted from a warmer growth temperature to a cooler growth temperature, it compensates by:
TLS Online TPP Program
#Question id: 414
#I Life Science/ Life Sciences Group – I-V
The hydrolysis of phosphoenolpyruvate proceeds with a DG'° of about –62 kJ/mol. The greatest contributing factors to this reaction are the destabilization of the reactants by electostatic repulsion and stabilization of the product pyruvate by:
TLS Online TPP Program
#Question id: 19630
#SCPH05 I Biotechnology
Which of the following is the weakest base?
TLS Online TPP Program
#Question id: 8705
#SCPH05 I Biotechnology
If you were using cladistics to build a phylogenetic tree of cats, which of the following would be the best outgroup?
TLS Online TPP Program
#Question id: 13090
#SCPH06 I Botany
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
What is the Colony 3’s plasmid is;