TLS Online TPP Program

#Question id: 5218


Several of the different globin genes are expressed in humans, but at different times in development. What mechanism could allow for this?

#SCPH28 | Zoology
  1. exon shuffling

  2. intron activation

  3. pseudogene activation

  4. differential gene regulation over time

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TLS Online TPP Program

#Question id: 9195

#SCPH06 I Botany

Philippe Bouchet and colleagues conducted a massive survey of marine molluscs on the west coast of New Caledonia. For 20% of the total species that were located and identified, only a single specimen was found. What does that suggest about the diversity of molluscs in this area?

TLS Online TPP Program

#Question id: 810

#SCPH28 | Zoology

Which of the following statements concerning the process of spontaneous folding of proteins is false?

TLS Online TPP Program

#Question id: 10892

#SCPH06 I Botany

Considering environment and ecosystem, identify the INCORRECT statement

TLS Online TPP Program

#Question id: 13091

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
Which colony’s plasmid do you actually want to use for your studies?

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#Question id: 4799

#SCPH12 I Genetics

You set up a mapping cross involving your favorite gene (YFG) in Neurospora and determine the following octad types: 4:4 arrangement 83; 2:4:2 arrangement 7; 2:2:2:2 arrangement 10. What is the distance between your gene and the centromere?