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#Question id: 13058
#SCPH01 Biochemistry
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following true about real time PCR?
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#Question id: 9220
#SCPH28 | Zoology
Which of the following factors could cause the largest increase in the effective population size of a species?
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#Question id: 387
#SCPH05 I Biotechnology
For a weak acid with a pKa of 6.0, What would be the ratio of acid to salt at pH 5.
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#Question id: 8841
#SCPH06 I Botany
Adaptive radiations can be a direct consequence of four of the following five factors. Select the exception.
TLS Online TPP Program
#Question id: 3035
#I Life Science/ Life Sciences Group – I-V
The equation used to obtain bacterial population by binary fission is