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TLS Online TPP Program

#Question id: 12908

Which statement is incorrect about CRISPR?

#SCPH28 | Zoology
  1. It  was first identified as an adaptive immunity pathway in prokaryotes providing resistance to bacteriophage and analogous to the RNA interference process found in eukaryotes. 
  2. It does not utilize double-stranded sequence-specific breaks and does not  repair using HDR via homologous recombination.
  3. The system consists of two parts, a short synthetic guide RNA ( gRNA) and a nonspecific double-stranded endonuclease termed Cas9. 
  4. It is also possible to program Cas9 with multiple guide RNAs to allow multiplex site-specific editing for designing large deletions, inversions and translocations.
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TLS Online TPP Program

#Question id: 3681

#SCPH01 Biochemistry

 A mutated E. coli cell carries out and completes DNA replication, but the lagging strands are found to contain more errors than normal. These errors are not found evenly distributed throughout the lagging strands, but are found in clusters. Which enzymatic activity is missing from this cell?
TLS Online TPP Program

#Question id: 3681

#SCPH05 I Biotechnology

 A mutated E. coli cell carries out and completes DNA replication, but the lagging strands are found to contain more errors than normal. These errors are not found evenly distributed throughout the lagging strands, but are found in clusters. Which enzymatic activity is missing from this cell?
TLS Online TPP Program

#Question id: 3681

#SCPH06 I Botany

 A mutated E. coli cell carries out and completes DNA replication, but the lagging strands are found to contain more errors than normal. These errors are not found evenly distributed throughout the lagging strands, but are found in clusters. Which enzymatic activity is missing from this cell?
TLS Online TPP Program

#Question id: 3681

#SCPH28 | Zoology

 A mutated E. coli cell carries out and completes DNA replication, but the lagging strands are found to contain more errors than normal. These errors are not found evenly distributed throughout the lagging strands, but are found in clusters. Which enzymatic activity is missing from this cell?
TLS Online TPP Program

#Question id: 3682

#SCPH01 Biochemistry

A mutated strain of E. coli replicates DNA normally at 25°C, but replication stops immediately when the temperature is raised to 37°C. Which protein is likely to be non-functional at the higher temperature?
TLS Online TPP Program

#Question id: 3682

#SCPH05 I Biotechnology

A mutated strain of E. coli replicates DNA normally at 25°C, but replication stops immediately when the temperature is raised to 37°C. Which protein is likely to be non-functional at the higher temperature?