To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

#Question id: 4964

#SCPH06 I Botany

Which of the following statements provides the strongest evidence that prokaryotes evolved before eukaryotes?

#Question id: 4964

#SCPH28 | Zoology

Which of the following statements provides the strongest evidence that prokaryotes evolved before eukaryotes?

#Question id: 4965

#SCPH06 I Botany

What is thought to be the correct sequence of these events, from earliest to most recent, in the evolution of life on Earth?

1.  origin of mitochondria

2.  origin of multicellular eukaryotes

3.  origin of chloroplasts

4.  origin of cyanobacteria

5.  origin of fungal-plant symbioses

#Question id: 4965

#SCPH28 | Zoology

What is thought to be the correct sequence of these events, from earliest to most recent, in the evolution of life on Earth?

1.  origin of mitochondria

2.  origin of multicellular eukaryotes

3.  origin of chloroplasts

4.  origin of cyanobacteria

5.  origin of fungal-plant symbioses

#Question id: 4966

#SCPH06 I Botany

If it were possible to conduct sophisticated microscopic and chemical analyses of microfossils found in 3.2-billion-year-old stromatolites, then within such microfossils, one should be surprised to observe evidence of:

I. double-stranded DNA

II. a nuclear envelope

III. a nucleoid

IV. a nucleolus

V. nucleic acids

#Question id: 4966

#SCPH28 | Zoology

If it were possible to conduct sophisticated microscopic and chemical analyses of microfossils found in 3.2-billion-year-old stromatolites, then within such microfossils, one should be surprised to observe evidence of:

I. double-stranded DNA

II. a nuclear envelope

III. a nucleoid

IV. a nucleolus

V. nucleic acids