To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

#Question id: 5203

#SCPH06 I Botany

The biggest problem with the shotgun technique is its tendency to underestimate the size of the genome. Which of the following might best account for this?

#Question id: 5203

#SCPH28 | Zoology

The biggest problem with the shotgun technique is its tendency to underestimate the size of the genome. Which of the following might best account for this?

#Question id: 5204

#SCPH06 I Botany

Bioinformatics can be used to scan sequences for probable genes looking for start and stop sites for transcription and for translation, for probable splice sites, and for sequences known to be found in other known genes. Such sequences containing these elements are called

#Question id: 5204

#SCPH28 | Zoology

Bioinformatics can be used to scan sequences for probable genes looking for start and stop sites for transcription and for translation, for probable splice sites, and for sequences known to be found in other known genes. Such sequences containing these elements are called

#Question id: 5205

#SCPH06 I Botany

Why is it preferable to use large computers and databases in searching for individual genes, rather than testing each sequence for possible function?

#Question id: 5205

#SCPH28 | Zoology

Why is it preferable to use large computers and databases in searching for individual genes, rather than testing each sequence for possible function?