When in a gel more Joule heat is produced than can be dissipated, the temperature in the middle of the gel is higher than at the lateral edges. This causes higher mobility of the ions in the center, resulting in an uneven distribution of the zones. The zones bend up at the two lateral sides, causing

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#Question id: 2601

#SCPH01 Biochemistry

Condition of basal level transcription (BLT);

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#Question id: 7754

#SCPH01 Biochemistry

In autophagy, the endoplasmic reticulum (ER) first gives rise to a cup-shaped, membranous cisterna called the

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#Question id: 13093

#SCPH06 I Botany

You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                                    

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?

In this table, DV = digested vector. DYG = digested yeast genome.

 

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#Question id: 10903

#SCPH05 I Biotechnology

Materials translocated in the phloem, such as sucrose, amino acids, hormones and some inorganic ions. Some inorganic solutes that move in the phloem called mobile elements. In contrast, some are relatively immobile in the phloem.

A) Immobile elements

B) Mobile elements

i) potassium, magnesium, phosphate, and chloride

ii) caesium, zinc, magnesium and iron

iii) nitrate, calcium, sulfur, and iron

iv) potassium, calcium, cobalt and caesium

Which of the following are the correct match of mobile and immobile elements in the phloem?

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#Question id: 6956

#SCPH01 Biochemistry

The central nervous system is derived from _____, the axial skeleton is derived from _____, and the muscles of the trunk are derived from _____?