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TLS Online TPP Program

#Question id: 12754

A restriction enzyme cuts 1000bp away from the recognition site the restriction enzyme is most likely to be

#SCPH28 | Zoology
  1. Type 1
  2. Type 2
  3. Type 3
  4. Type 2s
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TLS Online TPP Program

#Question id: 14275

#SCPH05 I Biotechnology

Nicotiana tabacum cells are cultured to high density for production of polysaccharide gum. The reactor used is a stirred tank, containing initially 100 litres medium. The maximum specific growth rate of the culture is 0.18 d-1 and the yield of biomass from substrate is 0.5 g g-l. The concentration of growth-limiting substrate in the medium is 3% (w/v). The reactor is inoculated with 1.5 g l- 1 cells and operated in batch until the substrate is virtually exhausted; medium flow is then started at a rate of 4 l d-1. Fed-batch operation occurs under quasi-steady-state conditions. Fed-batch operation is carried out for 40 d. What is the final mass of cells in the reactor?

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TLS Online TPP Program

#Question id: 5530

#I Life Science/ Life Sciences Group – I-V

 The cavity inside the blastula is called the
TLS Online TPP Program

#Question id: 19677

#SCPH05 I Biotechnology

For which of the following is the Hinsberg approach used?
TLS Online TPP Program

#Question id: 13088

#SCPH28 | Zoology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
What is the Colony 1’s plasmid is;
TLS Online TPP Program

#Question id: 2313

#SCPH06 I Botany

In which of the following cells would you expect to find the most mitochondria?