During your study of the gene for an enzyme you have isolated an amber mutation in the middle of the gene which produces a truncated form of the enzyme. By placing a +1 frameshift mutation a short distance before the amber mutation and a –1 frameshift mutation a short distance after the amber mutation you create a triple mutant that restores expression of a functional, full-length enzyme. 

a) +1 frameshift puts the amber mutation out of frame so that it is no longer read as a stop.

b) -1 frameshift is inserted after the amber so that no longer read as a stop.

c)  -1 frameshift is inserted after the amber to restore the frame of the end of the protein.

d) +1 frameshift puts the amber mutation out of frame so that it restore the frame of the end of the protein.

Which of the following is the correct prediction about the frame shift mutation?

TLS Online TPP Program

#Question id: 13056

#SCPH28 | Zoology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

TLS Online TPP Program

#Question id: 13057

#SCPH01 Biochemistry

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

TLS Online TPP Program

#Question id: 13057

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

TLS Online TPP Program

#Question id: 13057

#SCPH28 | Zoology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

TLS Online TPP Program

#Question id: 13058

#SCPH01 Biochemistry

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

TLS Online TPP Program

#Question id: 13058

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?