Not present in all bacteria, this structure enables those that possess it to germinate after exposure to harsh conditions, such as boiling:

TLS Online TPP Program

#Question id: 19790

#Unit 13. Methods in Biology

What will be the major difference between the PCR and replication is?

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#Question id: 19791

#Unit 13. Methods in Biology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19840

#Unit 13. Methods in Biology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA Polymerase	Sources
A- Tma	i) Pyrococcus furiosus
B- Deep Vent™	ii) Pyrococcus woesi
C- Tli	iii) Thermotoga maritima
D- Pfu	iv) Pyrococcus sp.
E- Pwo	v) Thermococcus litoralis

TLS Online TPP Program

#Question id: 19841

#Unit 13. Methods in Biology

Which of the following is true regarding Reverse transcriptase ?

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#Question id: 19842

#Unit 13. Methods in Biology

Primer-Dimer leads to the formation of ___

a) Inter-complemetary primers

b) Hybrid 

c) Amplicons

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#Question id: 19843

#Unit 13. Methods in Biology

Real time PCR analyzed various steps such as__

a) Denaturation

b) Annealing

c) Phosphorylation

d) Amplification

e) Methylation

f) Extension

Which of the following steps are correctly analyzed by real time PCR?