PIN and ABCB transport proteins direct the auxin efflux component of polar auxin transport throughout the plant

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#Id: 4684

#Unit 13. Methods in Biology

Bromodeoxyuridine-Based Assays for Cell Division Use Antibodies to Detect Newly Synthesized DNA

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#Id: 2554

#Unit 13. Methods in Biology

Fluorescent probes are commonly used to detect rapid biochemical changes in single living cells

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#Id: 2555

#Unit 13. Methods in Biology

Fluorescent probes are derived from a naturally occurring fluorescent protein, the green fluorescent protein (GFP) of the jellyfish Aequorea Victoria.

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#Id: 2556

#Unit 13. Methods in Biology

When excited by absorption of a photon of light, GFP emits a photon (that is, it fluoresces) in the green region of the spectrum. 

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#Id: 2557

#Unit 13. Methods in Biology

The light-absorbing/ emitting center of GFP (its chromophore) comprises an oxidized form of the tripeptide –Ser65–Tyr66–Gly67

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#Id: 2558

#Unit 13. Methods in Biology

This protein, from the bioluminescent jellyfish  Aequorea victoria, fluoresces with a peak wavelength of 508 nm (green light) when irradiated by ultraviolet or blue light (optimally 400 nm).