Nurturing Life Sciences
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#Unit 3. Fundamental Processes
In dam- E. coli If the plasmid is methylated, it undergoes a single round of replication, and if it is hemimethylated products accumulate
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The GATC sites at the origin remain hemimethylated for 13 minutes after replication.
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DNA methylation in bacteria allows the DNA mismatch recognition machinery to distinguish the old template strand from the new strand.
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Initiation of replication in E.Coli at oriC starts with formation of a complex that ultimately requires six proteins: DnaA, DnaB, DnaC, HU, gyrase, and SSB
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The minimal origin is defined by the distance between the outside members of the 13-mer and 9-mer repeats.
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The four 9-bp consensus sequences on the right side of oriC provide the initial binding sites for DnaA-ATP