TLS Online TPP Program

#Id: 4256

#Unit 12. Applied Biology

Passaging, is the removal of the medium and transfer of cells from a previous culture into fresh growth medium, a procedure that enables the further propagation of the cell line or cell strain.

TLS Online TPP Program

#Id: 4262

#Unit 12. Applied Biology

Characteristic Growth Pattern of Cultured Cells

TLS Online TPP Program

#Id: 4267

#Unit 12. Applied Biology

Trypsin is a serine protease that digest the extracellular protein or matrix protein so that cells get free.

TLS Online TPP Program

#Id: 4272

#Unit 12. Applied Biology

Dissociation of Adherent Cells

TLS Online TPP Program

#Id: 4274

#Unit 12. Applied Biology

To check the cell viability in cell suspension, trypan blue dye is used. This dye is excluded by viable cells but taken up by dead cells (nonviable cells).

TLS Online TPP Program

#Id: 4279

#Unit 12. Applied Biology

Cell viability is calculated as the number of viable cells divided by the total number of cells.