Nurturing Life Sciences
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#Unit 13. Methods in Biology
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Frequency of recognition site depends on A/T::G/C ratio of genome.
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The nuclear genome of mammals is 40% G+C and the dinucleotide CG is fivefold less common than statistically expected.
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CCG and CGG are the rarest trinucleotides in most A+T-rich bacterial genomes and CTAG is the rarest tetranucleotide in G+C-rich bacterial genomes.
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Recognize the same sequence and cut at exactly the same position, these enzymes are called isoschizomers.