C8 having two peptide chains: C8β and C8αϒ. Binding of C8β with C5b67 complex causes conformational change in the C8 dimer such that hydrophobic domain of C8αϒ can insert into the interior of the phospholipid membrane.

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#Unit 13. Methods in Biology

A. G. Tiselius (1937) Performed Moving boundary electrophoresis  in a U-shaped tube using Schlieren optics on both end for Measurements of the electrophoretic mobilities.

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#Unit 13. Methods in Biology

He received Nobel Prize in 1948 for developing technique for electrophoretic separation of substances.

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#Unit 13. Methods in Biology

Overall, there are three pillars electrophoresis

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#Unit 13. Methods in Biology

Medium - (buffer/liquid) - with a precise pH value and a constant ionic strength.

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#Unit 13. Methods in Biology

Overall, there are three pillars electrophoresis

Current / voltage

Medium - (buffer/liquid) - with a precise pH value and a constant ionic strength.

 Analytes - A compound, which is to be detected or assayed in a given sample.

Electrophoretic resolution mainly can be change by manipulating current and buffer system.

The higher the buffer concentration, the more electric power is applied, resulting in higher heat (joule effect) development.

Smiling Effect, Venetian Blind Effect & Electroendosmosis reduces the efficiency of electrophoresis.

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#Unit 13. Methods in Biology

Slab gels can develop a “Smiling Effect” When in a gel more Joule heat is produced than can be dissipated, leads to the higher mobility of the ions in the center, resulting in an uneven distribution of the zones, These zones bend up at the two lateral sides, causing “smiling.”