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TLS Online TPP Program

#Id: 1722

The α chains of the FcεRI, members of the immunoglobulin superfamily, directly bind the IgE heavy chains, whereas the β and γ chains are responsible for signal transduction.

#Unit 4. Cell Communication and Cell Signaling #Hypersensitivity and autoimmunity #Part B Pointers
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TLS Online TPP Program

#Id: 4672

#Unit 13. Methods in Biology

A precipitation curve for a system of one antigen and its antibodies. This plot of the amount of antibody precipitated versus increasing antigen concentrations (at constant total antibody) reveals three zones: 
TLS Online TPP Program

#Id: 4673

#Unit 13. Methods in Biology

A related quantitative technique, rocket electrophoresis, does permit measurement of antigen levels. In rocket electrophoresis, a negatively charged antigen is electrophoresed in a gel containing antibody. The precipitate formed between antigen and antibody has the shape of a rocket, the height of which is proportional to the concentration of antigen in the well. 
TLS Online TPP Program

#Id: 4674

#Unit 13. Methods in Biology

One limitation of rocket electrophoresis is the need for the antigen to be negatively charged for electrophoretic movement within the agar matrix
TLS Online TPP Program

#Id: 4675

#Unit 13. Methods in Biology

The interaction between antibody and a particulate antigen results in visible clumping called agglutination. Antibodies that produce such reactions are called agglutinins. 
TLS Online TPP Program

#Id: 4676

#Unit 13. Methods in Biology

Agglutination reactions are similar in principle to precipitation reactions they depend on the crosslinking of polyvalent antigens. Just as an excess of antibody inhibits precipitation reactions, such excess can also inhibit agglutination reactions; this inhibition is called the prozone effect.
TLS Online TPP Program

#Id: 4677

#Unit 13. Methods in Biology

A modification of the agglutination reaction, called agglutination inhibition, provides a highly sensitive assay for small quantities of an antigen. For example, one of the early types of home pregnancy test kits included latex particles coated with human chorionic gonadotropin (HCG) and antibody to HCG.