TLS Online TPP Program

#Id: 916

#Unit 13. Methods in Biology

Propidium iodide is a fluorescent dye with a flat, planar structure that slides between the rungs of (or intercalates into) the DNA ladder in a quantitative manner frequently used in FACS. 

TLS Online TPP Program

#Id: 917

#Unit 13. Methods in Biology

Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle.

TLS Online TPP Program

#Id: 918

#Unit 13. Methods in Biology

Propidium iodide-labeled cells in order as

1. Apoptotic cells

2. G1/G0

3. S

4. G2/M  

TLS Online TPP Program

#Id: 919

#Unit 13. Methods in Biology

Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).

TLS Online TPP Program

#Id: 920

#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell 

TLS Online TPP Program

#Id: 921

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles