Phosphorylation on Ser2 by P-TEFb activates elongation, splicing and polyadenylation

TLS Online TPP Program

#Id: 4230

#Unit 12. Applied Biology

Primary cultures are cells derived directly from tissues following enzymatic dissociation or from tissue fragments referred to as explants.

TLS Online TPP Program

#Id: 4231

#Unit 12. Applied Biology

Continuous cell lines are the cell lines that has gained the ability for infinite growth after transformation, which can occur spontaneously or can be chemically or virally induced. 

TLS Online TPP Program

#Id: 4233

#Unit 12. Applied Biology

TLS Online TPP Program

#Id: 4237

#Unit 12. Applied Biology

Hayflick limit of animal cell culture

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#Id: 4240

#Unit 12. Applied Biology

Most cells are anchorage dependent and must be cultured while attached to a solid or semi-solid substrate (adherent or monolayer culture), while others can be grown floating in the culture medium (suspension culture). 

TLS Online TPP Program

#Id: 4243

#Unit 12. Applied Biology

For optimal cell growth, a constant temperature of 370C and 5–10% CO2 is required for incubators to maintained physiological pH (usually pH 7.2–7.4).