During electrophoresis generation of heat is the major problem which leads to

ncreased rate of diffusion of sample and buffer ions.

Thermal instability which include denaturation of proteins.

A decrease of buffer viscosity.

Convection current leads mixing of separated samples

TLS Online TPP Program

#Id: 629

#Unit 3. Fundamental Processes

Replacement of Purine to Purine is called Transition

TLS Online TPP Program

#Id: 630

#Unit 3. Fundamental Processes

Replacement of Pyrimidine to Purine or vice versa is called Transversion.

TLS Online TPP Program

#Id: 631

#Unit 3. Fundamental Processes

Transition takes place due to Tautomeric Shift in Nitrogenous bases Spontaneously.

TLS Online TPP Program

#Id: 632

#Unit 3. Fundamental Processes

Transversion takes place due to Oxidation of Nitrogenous bases especially of Guanine due to reactive oxygen species.

TLS Online TPP Program

#Id: 633

#Unit 3. Fundamental Processes

Transition and Transversion showed up during replication only.

TLS Online TPP Program

#Id: 634

#Unit 3. Fundamental Processes

HNO₂ or Nitrous Acid is deaminating agent causes removal of –NH2 for nitrogenous bases.