Nurturing Life Sciences
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#Unit 13. Methods in Biology
In the PCR denaturation temperature calculated by using the Wallace rule, which is, Tm = 4 × (number of GC base pairs) + 2 × (number of AT base pairs).
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The size of a PCR product is mainly determined by the binding location of the PCR primers.
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Size of the primer negatively affects the efficiency of PCR.
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PCR amplification is mathematically counted as 2n So 10 cycle leads to 1024 times amplification
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