SDS (H3C-(CH2 )10 –CH2 -OSO3 – Na+ ) is an anionic detergent used to create negative on sample  On average, one SDS molecule binds for every two amino-acid residues.

TLS Online TPP Program

#Id: 900

#Unit 13. Methods in Biology

T4 polynucleotide kinase (PNK) Transfers terminal phosphate groups from ATP to 5-OH groups.

TLS Online TPP Program

#Id: 901

#Unit 13. Methods in Biology

Alkaline phosphatase Removes 5’ phosphates from DNA and RNA.

TLS Online TPP Program

#Id: 902

#Unit 13. Methods in Biology

Flow cytometry or FACS is the sine qua non (without which, nothing) of the modern immunologist’s toolbox.

TLS Online TPP Program

#Id: 903

#Unit 13. Methods in Biology

Flow cytometry is based on light scattering and fluorescence 

TLS Online TPP Program

#Id: 904

#Unit 13. Methods in Biology

Forward light scattering detect shape and side scattering detects granularity and complexity of the cell

TLS Online TPP Program

#Id: 905

#Unit 13. Methods in Biology

Hydrodynamically focused stream separate cells in single file