More recently Different from conventional gels, the matrix of single gel system introduced  which contains  three amino acids (serine, glycine and asparagine).

TLS Online TPP Program

#Id: 5064

#Unit 3. Fundamental Processes

13 minutes delaying, sequesters Oric regions in a form in which they are inaccessible to the Dam methylase and this is done by SeqA protein.

TLS Online TPP Program

#Id: 5065

#Unit 3. Fundamental Processes

SeqA mutants reduce the delay in remethylation at both oriC and dnaA. As a result, they initiate DNA replication too soon, thereby accumulating an excessive number of origins.

TLS Online TPP Program

#Id: 5066

#Unit 3. Fundamental Processes

SeqA is part of a negative regulatory circuit that prevents origins from being remethylated.

TLS Online TPP Program

#Id: 5067

#Unit 3. Fundamental Processes

Mutations in dnaA render replication asynchronous, which suggests that DnaA could be the “titrator” or “clock” that measures the number of origins relative to cell mass.

TLS Online TPP Program

#Id: 5068

#Unit 3. Fundamental Processes

dat locus is regulatory

In particular, a locus called dat has a large concentration of DnaA-binding sites. It binds about 80 more DnaA molecules than the origin.

Deletion of dat causes initiation to occur more frequently. This significantly increases the amount of DnaA available to the origin.

TLS Online TPP Program

#Id: 5069

#Unit 3. Fundamental Processes

DnaA promotes replication

SeqA delays new round of replication by inhibiting dam methylase binding

Dam methylase provide substrate for DnaA