Isoelectric Focusing Gels is based on the separation of molecules according to their different isoelectric points at which the show zero electrophoretic mobility.

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#Id: 7601

#Unit 5. Developmental Biology

In one pathway, sperm that have undergone the acrosome reaction bind directly to ZP2

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#Id: 7602

#Unit 5. Developmental Biology

In 2nd pathway, sperm with intact acrosomes become bound to ZP3, which causes the acrosome reaction, and the sperm binding is then transferred to ZP2

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#Id: 7603

#Unit 5. Developmental Biology

Two important acrosomal proteins  acrosin and MMP2 (matrix metaloproteinase-2)

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#Id: 7604

#Unit 5. Developmental Biology

MMP2 is a protein that digests extracellular matrices (such as the zona) and may be responsible for digesting a pathway to the oocyte Acrosin bind secondarily to the zona, keeping the sperm in the channel it creates

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#Id: 7605

#Unit 5. Developmental Biology

The acrosome reaction, exposes the inner acrosomal membrane to the outside. The junction between this inner acrosomal membrane and the sperm cell membrane is called the equatorial region, and this is where membrane fusion between sperm and egg begins

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#Id: 7606

#Unit 5. Developmental Biology

Gamete fusion involve proteins: Izumo, originally in the acrosomal membrane Juno, an oocyte membrane protein