Cellulose Acetate Electrophoresis have clinical role for example enzyme and antibodies are easily detected by using Cellulose Acetate Electrophoresis with zymogen technique.

TLS Online TPP Program

#Id: 2993

#Unit 3. Fundamental Processes

The first DNA-synthesizing enzyme to be characterized was DNA polymerase I, which is a single polypeptide of 103 kD, The chain can be cleaved into two parts by proteolytic treatment.

The larger cleavage product (68 kD) is called the Klenow fragment. It is used in synthetic reactions in vitro, It contains the polymerase and the proofreading 3'-5'  exonuclease activities.

The small fragment (35 kD) possesses a 5 '-3' exonucleolytic activity, which excises small groups of nucleotides, up to - 10 bases at a time. This activity is coordinated with the synthetic/ proofreading activity. It provides DNA polymerase I with a unique ability to start replication in vitro at a nick in DNA.

TLS Online TPP Program

#Id: 2994

#Unit 3. Fundamental Processes

Nick translation has been a major technique for introducing radioactively labeled nucleotides into DNA in vitro.

TLS Online TPP Program

#Id: 2996

#Unit 3. Fundamental Processes

The coupled 5'-3' synthetic/ 3'-5' exonucleolytic action is used most extensively for filling in short single-stranded regions in double-stranded DNA. These regions arise during lagging strand DNA replication.

TLS Online TPP Program

#Id: 2997

#Unit 3. Fundamental Processes

These Okazaki fragments are found in replicating DNA in both prokaryotes and eukaryotes.

TLS Online TPP Program

#Id: 2998

#Unit 3. Fundamental Processes

On the leading strand (sometimes called the forward strand) DNA synthesis can proceed continuously in the 5 ' to 3’ direction as the parental duplex is unwound.

TLS Online TPP Program

#Id: 3005

#Unit 3. Fundamental Processes

On the lagging strand a stretch of single-stranded parental DNA must beexposed, and then a segment is synthesized in the reverse direction (relative to fork movement).