The duration of annealing step is usually 1 min during the first as well as the subsequent cycles of PCR. 

TLS Online TPP Program

#Id: 3634

#Unit 3. Fundamental Processes

When inosine (I) is the first (wobble) nucleotide of an anticodon, three different codons can be recognized—the maximum number for any tRNA.

TLS Online TPP Program

#Id: 3635

#Unit 3. Fundamental Processes

A minimum of 32 tRNAs are required to translate all 61 codons (31 to encode the amino acids and 1 for initiation).

TLS Online TPP Program

#Id: 3636

#Unit 3. Fundamental Processes

Rate of protein synthesis slows down when all three bases of a codon engaged in strong Watson-Crick pairing with the three bases of the anticodon.

TLS Online TPP Program

#Id: 3637

#Unit 3. Fundamental Processes

Normally there are 20 AATS exclusively specific for specific amino acid.

TLS Online TPP Program

#Id: 3638

#Unit 3. Fundamental Processes

There are two class of AATS.
1.Class I – that transfer amino acid at 2’ OH and then 3’ OH &
2. Class II – that directly transfer the amino acid to 3’ OH

TLS Online TPP Program

#Id: 3639

#Unit 3. Fundamental Processes

Class I AATS are generally monomer and rarely dimer.