Nurturing Life Sciences
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The specificity of the PCR depends crucially upon the primers. The following factors are important in choosing effective primers
Primers should be 17 to 30 nucleotides in length
A GC content of about 50% is ideal. For primers with a low GC content, it is desirable to choose a long primer to avoid a low melting
temperature.
Sequences with long runs (i.e., more than three or four) of a single nucleotide should be avoided.
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#Unit 10. Ecological Principles
Restoration ecology is the full or partial repair or replacement of biological habitats and/or their populations that have been degraded or destroyed.
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Complete restoration - Conservationists attempt to return a habitat to its condition prior to the disturbance.
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Ecosystem replacement - replaces the original community with a different one
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Many restored habitats have greater biodiversity and ecosystem services than degraded habitats.
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