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An excited fluorescent molecule such as GFP or YFP can dispose of the energy from the absorbed photon in either of two ways: 


by fluorescence, emitting a photon of slightly longer wavelength (lower energy) than the exciting light, or 


by nonradiative fluorescence resonance energy transfer (FRET).




#Unit 13. Methods in Biology #Fluorescence #Part B Pointers
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#Unit 1. Molecules and their Interaction Relevant to Biology

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#Unit 1. Molecules and their Interaction Relevant to Biology

The cytosolic isozyme has two distinct functions. It catalyzes the conversion of citrate to isocitrate, providing the substrate for a cytosolic isocitrate dehydrogenase that generates NADPH as reducing power for fatty acid synthesis and other anabolic processes in the cytosol. It also has a role in cellular iron homeostasis.

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#Unit 1. Molecules and their Interaction Relevant to Biology

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