One simply harvests cellular RNA containing the transcript whose 59-end is to be mapped and whose sequence is known. 

TLS Online TPP Program

#Id: 8959

#Unit 11. Evolution and Behavior

Intimacy between the genomes of the mitochondria and the nucleus within a cell, it may not be surprising that extensive HGT has occurred between them

TLS Online TPP Program

#Id: 6136

#Unit 3. Fundamental Processes

XPC detect distortions = UvrA in E. coli

Formation of the bubble involves the helicase activities of the proteins XPA and XPD (the equivalent to UvrB in E. coli) and the single-strand-binding protein RPA. The bubble creates cleavage sites 5’ to the lesion for a nuclease known as ERCC1-XPF and 3’ to the lesion for nuclease XPG (representing the function of UvrC). In higher cells, the resulting DNA strand is 24–32 nucleotides long. As in bacteria, the DNA strand is released to create a gap that is filled in by the action of DNA polymerase and ligase.

XPB and XPD are both helicases; the XPB helicase is required for promoter melting during transcription, while the XPD helicase performs the unwinding function in NER

TLS Online TPP Program

#Id: 4950

#Unit 6. System Physiology – Plant

PHYA is the only gene that encodes a Type I phytochrome. Phytochrome A predominantly perceives far-red light.

TLS Online TPP Program

#Id: 7058

#Unit 11. Evolution and Behavior

The Forces of Evolution

mutation, 

genetic drift

gene flow or migration

selection.

TLS Online TPP Program

#Id: 8774

#Unit 1. Molecules and their Interaction Relevant to Biology

The Cp heat capacity of a defined system is the amount of heat (usually expressed in calories, kilocalories, or joules) needed to raise the system's temperature by one degree (usually expressed in Celsius or Kelvin).