We perform DNase footprinting by binding the protein to its DNA target, then digesting the DNA–protein complex with DNase. 

When we electrophorese the resulting DNA fragments, the protein binding site shows up as a gap, or “footprint,” in the pattern where the protein protected the 

DNA from degradation.

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#Unit 4. Cell Communication and Cell Signaling

C5b component is extremely labile, is not covalently bound to the membrane like C3b and C4b, and is rapidly inactivated unless it is stabilized by the binding of C6.

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#Id: 749

#Unit 3. Fundamental Processes

A common method to monitor synthesis of new DNA is Incorporation Assays.

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#Id: 6290

#Unit 3. Fundamental Processes

Consensus Sequences

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#Id: 2505

#Unit 13. Methods in Biology

IEF method is particularly useful for separating isoenzymes, which are different forms of the same enzyme, often differing by only one or two amino-acid residues.

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#Id: 5202

#Unit 7. System Physiology – Animal

Hemophilia A, is caused by factor VIII deficiency.