HIC column is made up of phenyl-Sepharose, where phenyl groups are bonded to the insoluble support Sepharose.

TLS Online TPP Program

#Id: 8388

#Unit 2. Cellular Organization

Smooth muscle cells and nonmuscle cells are regulated by:

Norepinephrine, angiotensin, endothelin, histamine

TLS Online TPP Program

#Id: 8389

#Unit 2. Cellular Organization

Smooth muscle contraction is regulated by the cycling of myosin II between on and off states.

TLS Online TPP Program

#Id: 8390

#Unit 2. Cellular Organization

In vertebrate smooth muscle, phosphorylation of the myosin regulatory light chain activates contraction in Ca2+ dependent manner.

TLS Online TPP Program

#Id: 8391

#Unit 2. Cellular Organization

When the Ca2+ level rises, Ca2+ binds CaM.

TLS Online TPP Program

#Id: 8392

#Unit 2. Cellular Organization

The CaM*-Ca2+ complex binds and activates MLC kinase, phosphorylates the myosin LC. 

TLS Online TPP Program

#Id: 8393

#Unit 2. Cellular Organization

Phosphorylation of MLC 

unfolds the myosin II, active and can assemble into bipolar filaments for contraction