Flow cytometry or FACS is the sine qua non (without which, nothing) of the modern immunologist’s toolbox.

TLS Online TPP Program

#Id: 3122

#Unit 3. Fundamental Processes

oriC also contains 11 GATC/CTAG repeats that are methylated on adenine on both strands.

TLS Online TPP Program

#Id: 3123

#Unit 3. Fundamental Processes

Replication generates hemimethylated DNA, which cannot initiate replication

TLS Online TPP Program

#Id: 3124

#Unit 3. Fundamental Processes

There is a 13-minute delay before the GATC/CTAG repeats are remethylated. 

TLS Online TPP Program

#Id: 3126

#Unit 3. Fundamental Processes

oriC contains binding sites for DnaA: dnaA-boxes.DnaA is an ATP-bindingprotein and its binding to DNA is affected depending on whether ATP, ADP, or no nucleotide is bound.

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#Id: 3127

#Unit 3. Fundamental Processes

One mechanism by which the activity of the replication origin is controlled is DNA methylation. 

TLS Online TPP Program

#Id: 3128

#Unit 3. Fundamental Processes

The E. coli oriC contains 11 copies of the sequence GATC/CTAG which is a target for methylation at the N6 position of adenine by the Dam methylase enzyme.