In Ion-exchange chromatography There are two types of ion exchanger, namely cation  and anion exchangers.

TLS Online TPP Program

#Id: 349

#Unit 1. Molecules and their Interaction Relevant to Biology

The oxaloacetate is converted to PEP by phosphoenolpyruvate carboxykinase. This Mg2+ dependent reaction requires GTP as the phosphoryl group donor.

TLS Online TPP Program

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#Unit 1. Molecules and their Interaction Relevant to Biology

Transketolase requires the cofactor thiamine pyrophosphate (TPP)

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#Id: 354

#Unit 1. Molecules and their Interaction Relevant to Biology

Acetyl-CoA and NADH are potent allosteric inhibitors of the enzyme. 

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#Unit 1. Molecules and their Interaction Relevant to Biology

Acetyl-CoA inhibits the transacetylase component whereas NADH inhibits the dihydrolipoyl dehydrogenase component. 

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#Unit 1. Molecules and their Interaction Relevant to Biology

The reaction catalyzed by citrate synthase is essentially a Claisen condensation, involving a thioester (acetyl-CoA) and a ketone (oxaloacetate)

TLS Online TPP Program

#Id: 359

#Unit 1. Molecules and their Interaction Relevant to Biology

Eukaryotic cells have two isozymes of aconitase

• The mitochondrial isozyme (convert citrate to isocitrate in the citric acid cycle)

• The cytosolic isozyme