Affinity chromatography fraction can be analyzed by two ways

Absorption or UV visible spectrophotometry which analyzes the concentration of protein in the fraction by using Beer lamberts law.

Polyacrylamide gel electrophoresis to fractionize the protein sample on the basis of their molecular weight.

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#Unit 8. Inheritance Biology

Prototrophs– Wild type

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Auxotrophs- Nutritional mutant that disrupts the ability to synthesize an essential biological molecule = mutant type

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Auxotrophs or Mutant bacterial strains can be isolated on the basis of their nutritional requirements.

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Plasmid Small, circular DNA molecule found in bacterial cells that is capable of replicating independently from the bacterial chromosome.

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Episome Plasmid capable of integrating into a bacterial chromosome.

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#Unit 8. Inheritance Biology

F (fertility) factor Episome of E. coli that controls conjugation and gene exchange between cells.