Affinity chromatography fraction can be analyzed by two ways

Absorption or UV visible spectrophotometry which analyzes the concentration of protein in the fraction by using Beer lamberts law.

Polyacrylamide gel electrophoresis to fractionize the protein sample on the basis of their molecular weight.

TLS Online TPP Program

#Id: 6663

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6664

#Unit 3. Fundamental Processes

Splicing is an SN2 reaction that proceeds through a pentavalent phosphorous intermediate.

TLS Online TPP Program

#Id: 6665

#Unit 3. Fundamental Processes

There are three type of Splicing Machinery

TLS Online TPP Program

#Id: 6666

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6667

#Unit 3. Fundamental Processes

The group I and group II introns have the remarkable ability to excise themselves from an RNA. This is called autosplicing, or self splicing. 

Group I introns are more common than group II introns.

TLS Online TPP Program

#Id: 6668

#Unit 3. Fundamental Processes

Two transesterification reactions takes place for 2 exon -1 intron configuration. First transesterification is catalysed variably either by external G or branch point while second transesterification is always caused by exon 1 -3’OH.