individual replicons in eukaryotic genomes are relatively small, typically -40 kb in yeast or flies and -100 kb in animal cells.

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#Unit 1. Molecules and their Interaction Relevant to Biology

Similarly, if the inhibitor binds to a different site from the substrate and reduces the affinity of the enzyme for the substrate without altering the reaction characteristics of that substrate which does bind, then hyperbolic competitive inhibition results

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#Unit 1. Molecules and their Interaction Relevant to Biology

Dixon plot for competitive inhibition

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#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

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#Unit 1. Molecules and their Interaction Relevant to Biology

Uncompetitive inhibitors bind only to the enzyme-substrate complex and not to the free enzyme. 

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#Unit 1. Molecules and their Interaction Relevant to Biology

In neither case does the inhibitor compete with the substrate for the same binding site, so the inhibition cannot be overcome by increasing the substrate concentration.

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#Unit 1. Molecules and their Interaction Relevant to Biology

Substrate-binding could cause a conformational change to take place in the enzyme and reveal an inhibitor binding site, or the inhibitor could bind directly to the enzyme-bound substrate.