DNA ligase makes the bond that connects the 3 ' end of one Okazaki fragment to the 5 ' beginning of the next fragment.

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#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits, Rpb1 and Rpb2

a DNA template, whereas in eukaryotes, transcription takes place on a chromatin template.

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#Unit 3. Fundamental Processes

Eukaryotic nuclei contain RNA polymerase I, RNA polymerase II, and RNA polymerase III can be separated by using DEAE-Sephadex ion-exchange chromatography

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#Unit 3. Fundamental Processes

RNA Polymerase can also separated by using epitope tagging. Normally RPB3 is used to create epitope tag.

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#Id: 6457

#Unit 3. Fundamental Processes

Molecular genetic techniques are used to make a cDNA encoding a recombinant protein to which is fused a short sequence of amino acids called an epitope tagging.

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#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG, which encodes the amino acid sequence DYKDDDDK (single-letter amino acid code), and myc, which encodes the sequence EQKLISEEDL.

TLS Online TPP Program

#Id: 6459

#Unit 3. Fundamental Processes