A replication fork is initiated at the origin and then moves sequentially along DNA

TLS Online TPP Program

#Id: 747

#Unit 3. Fundamental Processes

A:T and G:C base pairs have same geometry that’s why DNA polymerase has only one catalytic site for polymerization 

TLS Online TPP Program

#Id: 748

#Unit 3. Fundamental Processes

Incorrect base pairing leads to dramatically lower rates of nucleotide addition called as kinetic proofreading

TLS Online TPP Program

#Id: 749

#Unit 3. Fundamental Processes

A common method to monitor synthesis of new DNA is Incorporation Assays.

TLS Online TPP Program

#Id: 750

#Unit 3. Fundamental Processes

rNTPs are present at approximately 10-fold higher concentration in the cell, they are incorporated at a rate that is more than 1000-fold lower than dNTPs.

TLS Online TPP Program

#Id: 751

#Unit 3. Fundamental Processes

The palm domain is composed of a b sheet and contains the primary elements of the catalytic site.

TLS Online TPP Program

#Id: 752

#Unit 3. Fundamental Processes

DNA polymerase binds two divalent metal ions (typically Mg2+ orZn2+) that alter the chemical environment around the correctly base-paired dNTP and the 3’-OH of the primer