When there are mtDNAs with allelic variations in the same cell, called heteroplasmy (either because of inheritance from different parents or because of mutation).

TLS Online TPP Program

#Id: 2816

#Unit 13. Methods in Biology

When in the protein solution divalent salts, or multivalent salts the protein separated in form of aggregate.

TLS Online TPP Program

#Id: 2817

#Unit 13. Methods in Biology

In the salt fractionation most polar will be fractionated with large amount of salt.

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#Id: 2818

#Unit 13. Methods in Biology

In the HIC some highly hydrophobic proteins may not even be eluted in the total absence of salt in such case water-miscible organic solvent such as propanol or ethylene glycol to the column buffer solution.

TLS Online TPP Program

#Id: 2819

#Unit 13. Methods in Biology

Ion-exchange chromatography relies on the attraction between oppositely charged stationary phase, known as an ion exchanger, and analyte.

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#Id: 2820

#Unit 13. Methods in Biology

Ion-exchange chromatography is frequently chosen for the separation and purification of proteins, peptides, nucleic acids, polynucleotides and other charged molecules, mainly because of its high resolving power and high capacity.

TLS Online TPP Program

#Id: 2821

#Unit 13. Methods in Biology

In Ion-exchange chromatography There are two types of ion exchanger, namely cation  and anion exchangers.