A typical gene transcribed by RNA polymerase II has a promoter that extends upstream from the site where transcription is initiated.

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#Id: 888

#Unit 13. Methods in Biology

The repeat region of the Huntington disease locus (HD) in six individuals is amplified by PCR and analyzed by gel electrophoresis as shown in the following figure; the numbers to the right indicate the sizes of the PCR products in bp.

               

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#Id: 889

#Unit 13. Methods in Biology

A recognition site frequency every 44 (256) bp, 46 (4096) bp and 48 (65 536) bp, if A=T=G=C.

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#Id: 890

#Unit 13. Methods in Biology

Frequency of recognition site depends on A/T::G/C ratio of genome.

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#Id: 891

#Unit 13. Methods in Biology

The nuclear genome of mammals is 40% G+C and the dinucleotide CG is fivefold less common than statistically expected.

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#Id: 892

#Unit 13. Methods in Biology

CCG and CGG are the rarest trinucleotides in most A+T-rich bacterial genomes and CTAG is the rarest tetranucleotide in G+C-rich bacterial genomes.

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#Id: 893

#Unit 13. Methods in Biology

RE’ has two functions-

a) Restriction of foreign genome

b) Modification/methylation of host genome using SAM