Mutations are also caused by compounds that substitute for normal bases (base analogs) or slip between the bases (intercalating agents) to cause errors in replication. 

TLS Online TPP Program

#Id: 917

#Unit 13. Methods in Biology

Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle.

TLS Online TPP Program

#Id: 918

#Unit 13. Methods in Biology

Propidium iodide-labeled cells in order as

1. Apoptotic cells

2. G1/G0

3. S

4. G2/M  

TLS Online TPP Program

#Id: 919

#Unit 13. Methods in Biology

Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).

TLS Online TPP Program

#Id: 920

#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell 

TLS Online TPP Program

#Id: 921

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles

TLS Online TPP Program

#Id: 922

#Unit 13. Methods in Biology

CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides.