DinB and UmuC are members of a distinct family of DNA polymerases found in many organisms known as the Y family of DNA polymerases. There are five translesion polymerases known in humans, four of which belong to the Y family. 

TLS Online TPP Program

#Id: 2631

#Unit 13. Methods in Biology

S1 mapping is used to locate the 5’- or 3’-ends of RNAs and to quantify the amount of a given RNA in cells at a given time. 

TLS Online TPP Program

#Id: 2632

#Unit 13. Methods in Biology

The principle behind the S1 mapping method is to label a single stranded DNA probe that can hybridize only to the transcript of interest. 

TLS Online TPP Program

#Id: 2633

#Unit 13. Methods in Biology

The probe must span the sequence where the transcript starts or ends. After hybridizing the probe to the transcript, one applies S1 nuclease, which degrades only single-stranded DNA and RNA. 

TLS Online TPP Program

#Id: 2634

#Unit 13. Methods in Biology

The probe must span the sequence where the transcript starts or ends. After hybridizing the probe to the transcript, one applies S1 nuclease, which degrades only single-stranded DNA and RNA.

TLS Online TPP Program

#Id: 2635

#Unit 13. Methods in Biology

Double-stranded DNAs, RNAs, and hybrids are protected from S1 nuclease degradation. 

TLS Online TPP Program

#Id: 2636

#Unit 13. Methods in Biology

S1 mapping the 5’-end of a transcript