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TLS Online TPP Program

#Id: 8687

#Unit 1. Structure and Function of Biomolecules #Metabolism #Part B Pointers
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TLS Online TPP Program

#Id: 8770

#Unit 1. Structure and Function of Biomolecules

                                                                                                                                                                                                                                                                      
TLS Online TPP Program

#Id: 8771

#Unit 1. Structure and Function of Biomolecules


TLS Online TPP Program

#Id: 8772

#Unit 1. Structure and Function of Biomolecules

van’t Hoff plot is used measure rate of unfolding of proteins
TLS Online TPP Program

#Id: 8773

#Unit 1. Structure and Function of Biomolecules

Techniques for Measuring Stability

Absorbance (e.g. Trp, Tyr) 

Fluorescence (Trp)-difference in emission max & intensity.

CD (far or near UV) - (2o or 3o) 

NMR 

DSC (calorimetry) 

Urea gradient gels - difference in the migrating rates between F and U.

Catalytic activity 

Chromophoric or fluorophoric probes/FRET 
TLS Online TPP Program

#Id: 8774

#Unit 1. Structure and Function of Biomolecules

The Cp heat capacity of a defined system is the amount of heat (usually expressed in calories, kilocalories, or joules) needed to raise the system's temperature by one degree (usually expressed in Celsius or Kelvin).
TLS Online TPP Program

#Id: 8775

#Unit 1. Structure and Function of Biomolecules

Thermal stability of protein can be calculated