Careful measurements of the binding constants show that FoF1 binds ATP with very high affinity (K d < 10^-12 M) and ADP with much lower affinity (Kd ≈ 10^-5 M).

TLS Online TPP Program

#Id: 2788

#Unit 13. Methods in Biology

Therefore, that k is related to the distribution coefficient. Since amount and concentration are related by volume by

TLS Online TPP Program

#Id: 2789

#Unit 13. Methods in Biology

The second component is the time the stationary phase retains the analyte, referred to as the adjusted retention time, t’R.

TLS Online TPP Program

#Id: 2790

#Unit 13. Methods in Biology

The ratio VS/VM is referred to as the volumetric phase ratio, b. Hence:

TLS Online TPP Program

#Id: 2791

#Unit 13. Methods in Biology

notSelectivity or separation factor, a, which can also be viewed as simply the relative retention ratio for the two analytes:

TLS Online TPP Program

#Id: 2792

#Unit 13. Methods in Biology

Chromatography columns are considered to consist of a number of adjacent zones in each of which there is sufficient space for an analyte to completely equilibrate between the two phases, Each zone is called a theoretical plate (having no N).

TLS Online TPP Program

#Id: 2793

#Unit 13. Methods in Biology

The length of column containing one theoretical plate is referred to as the plate height, H, which has units of length normally in micrometers.