There are 6000 genes in yeast; 21,700 in a nematode worm; 17,000 in a fly; 25,000 in the small plant Arabidopsis; and probably 20,000 to 25,000 in mice and humans

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#Id: 2858

#Unit 13. Methods in Biology

Destructive method uses Folin reagent, Biuret, BCA, Coomassie Brilliant Blue and give absorbance at 660, 540, 562, 595 nm of wavelength respectively.

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#Id: 2859

#Unit 13. Methods in Biology

The constructive method involved direct analysis of protein and gives absorbance at wavelength of 280 nm.

TLS Online TPP Program

#Id: 2860

#Unit 13. Methods in Biology

Carotenoids gives absorbance between 400 – 500 nm of wavelength while porphyrin gives absorbance at 400 nm of wavelength known as Soret band.

TLS Online TPP Program

#Id: 2861

#Unit 13. Methods in Biology

DNA gives absorbance at 595 nm in presence of Diphenylamine while gives direct absorbance at 260 nm

TLS Online TPP Program

#Id: 2862

#Unit 13. Methods in Biology

RNA gives absorbance at 595 nm in presence of Diphenylamine while gives direct absorbance at 540 nm.

TLS Online TPP Program

#Id: 2863

#Unit 13. Methods in Biology

The electronic transitions of the peptide bond occur in the far UV. The intense peak at 190 nm, and the weaker one at 210–220 nm is due to the p ® p* and n ® p* transitions.