Nurturing Life Sciences
#Id: 378
Topological states of covalently closed, circular (ccc) DNA.
#Id: 2627
#Unit 13. Methods in Biology
Types and examples of typical enzymes used in the manipulation of nucleic acids
#Id: 2628
#Id: 2629
End-labelling of a gene probe at the 5′ end with alkaline phosphatase and polynucleotide kinase.
#Id: 2630
End-labelling of a gene probe at the 3′ end using terminal transferase. Note that the addition of a labeled dNTP at the 3′ end alters the sequence of the gene probe.
#Id: 2631
S1 mapping is used to locate the 5’- or 3’-ends of RNAs and to quantify the amount of a given RNA in cells at a given time.
#Id: 2632
The principle behind the S1 mapping method is to label a single stranded DNA probe that can hybridize only to the transcript of interest.
