H3.3 is often referred to as a “replacement” histone.

TLS Online TPP Program

#Id: 8542

#Unit 1. Molecules and their Interaction Relevant to Biology

If the concentration of the enzyme or substrate is increased, the rate of the reaction will be increased as well. 

If the temperature of the enzymes surrounding environment is increased, then the rate of the reaction will also be increased. 

If the substrate concentration is increased then the rate of the reaction will increase. 

If an inhibitor is added then the rate of the reaction will be decreased significantly

TLS Online TPP Program

#Id: 8543

#Unit 1. Molecules and their Interaction Relevant to Biology

One drawback from the Eadie–Hofstee approach is that neither ordinate nor abscissa represent independent variables: both are dependent on reaction rate. Thus any experimental error will be present in both axes.

TLS Online TPP Program

#Id: 8544

#Unit 1. Molecules and their Interaction Relevant to Biology

The abscissa refers to the (x) coordinate and the ordinate refers to the (y) coordinate of a standard two-dimensional graph.

TLS Online TPP Program

#Id: 8545

#Unit 1. Molecules and their Interaction Relevant to Biology

Irreversible inhibitors, such as the organophosphorus and organomercury compounds, cyanide, carbon monoxide and hydrogen sulphide, combine with the enzyme to form a covalent bond. 

TLS Online TPP Program

#Id: 8546

#Unit 1. Molecules and their Interaction Relevant to Biology

The extent of irreversible inhibition of the enzyme is dependent upon the reaction rate constant (and hence time) for covalent bond formation and upon the amount of inhibitor present.

TLS Online TPP Program

#Id: 8547

#Unit 1. Molecules and their Interaction Relevant to Biology

The irreversible inhibitors cannot be removed by simple physical techniques such as dialysis, is to reduce the amount of enzyme available for reaction.