Proteins that contain prosthetic groups (e.g. haem, flavin, carotenoid) and some metal–protein complexes, may have strong absorption bands in the UV/Vis range.

TLS Online TPP Program

#Id: 4467

#Unit 13. Methods in Biology

The total magnification is a product of the magnification of the individual lenses.

Total magnification= 40X*10X

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#Id: 4468

#Unit 13. Methods in Biology

The smaller the value of D, the better the resolution. The value of D is given by the equation.

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#Id: 4469

#Unit 13. Methods in Biology

Resolving power or resolution is the ability to distinguish between two very closely positioned objects.

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#Id: 4470

#Unit 13. Methods in Biology

Resolution is improved by using shorter wavelengths of light (decreasing the value of λ) or by gathering more light (increasing either N or α).

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#Id: 4471

#Unit 13. Methods in Biology

The limit of resolution of a light microscope using visible light is about 0.2 μm (200 nm).

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#Id: 4472

#Unit 13. Methods in Biology

Phase contrast and Differential interference microscopy are used to image live cells and unstained tissues.