Destructive method uses Folin reagent, Biuret, BCA, Coomassie Brilliant Blue and give absorbance at 660, 540, 562, 595 nm of wavelength respectively.

TLS Online TPP Program

#Id: 1858

#Unit 3. Fundamental Processes

TATA less, Inr Less promoters uses Sp1 activator to position TBP with TAFs

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#Id: 1859

#Unit 3. Fundamental Processes

Sp1 (Mr  80,000) is a transcription activator for a large number of genes in higher eukaryotes. Its DNA binding site, the GC box consensus sequence GGGCGG.

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#Id: 1860

#Unit 3. Fundamental Processes

Sp1 contains three zinc fingers, 25% of their amino acid residues are Gln.

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#Id: 1861

#Unit 3. Fundamental Processes

RNA polymerase III transcribes a set of short genes. The classical class III genes (types I and II) have promoters that lie wholly within the genes.

 

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#Id: 1862

#Unit 3. Fundamental Processes

RNA Polymerase I has a Bipartite Promoter

 

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#Id: 1981

#Unit 3. Fundamental Processes

This structure is called a cap.