Thyrotropin-releasing hormone (TRH) Tripeptide (amide—pyroglutamyl-histidylproline-amide) Stimulates secretion of TSH by thyrotropes

TLS Online TPP Program

#Id: 6458

#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG, which encodes the amino acid sequence DYKDDDDK (single-letter amino acid code), and myc, which encodes the sequence EQKLISEEDL.

TLS Online TPP Program

#Id: 6459

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6460

#Unit 3. Fundamental Processes

Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell.

Alexa- 488-labeled antibody -emit green light

Alexa-568-labeled antibody- emit red light

TLS Online TPP Program

#Id: 6461

#Unit 3. Fundamental Processes

A significant difference between the transcription of eukaryotic and prokaryotic RNAs is that in bacteria, transcription takes place on  RNA polymerase III synthesizes small RNAs in the nucleoplasm

TLS Online TPP Program

#Id: 6462

#Unit 3. Fundamental Processes

A typical gene transcribed by RNA polymerase II has a promoter that extends upstream from the site where transcription is initiated.

TLS Online TPP Program

#Id: 6463

#Unit 3. Fundamental Processes

A regulatory site that binds more negative regulators than positive regulators to control transcription is called a silencer.